The amylase was incubated in a 9㎜ hole on the starch agar gel plate bored with a cork borer. When 0.1N-iodine solution sprayed on the plate, the formed uncolored zone were observed. An activity of amylase has been determined by measurement of diameter of the uncolored zone. We named this method $quot;cork borer method$quot;.
When amylase was incubated on the starch agar gel plate, the following points were obtained.
1) The optimum pH for the formation of the zone in case of amylases(Biotex, Spitase) which produced by Bacillus is neutrality and alkali, while that for Glucoamylase, Biodiase, and Mucorrennin which produced by Rhizopus and Mucor is from 5 to 7.
2) The diameter of the zone was increased by the incubation time and amylase activity.
3) The zone was easily formed at low level of starch concentration and was formed much bigger than at 50℃ than at 10℃.
From the above results, after malting the starch agar gel plate, keeping constant concentration of the starch, the measurement of amylase activity is in efficiency upon the constant reaction time and temperature.
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